Reported sensitivities and specificities of RT-QuIC in sCJD, using different rPrP substrates.1
| RT-QuIC | rPrP | Patient group (n) | Sensitivity (%) | Sensitivity in different PRNP codon 129 genotypes (%) | Controls (n) | Specificity (%) | Reference |
| First generation | Full-length human | Neuropathologically confirmed sCJD (34) | 85 | MM (86); MV (100); VV (75) | Neurodegenerative disease controls (165) | 100 | Atarashi et al6 |
| First generation | Full-length human | Neuropathologically confirmed and probable sCJD (81) | 77 | ND | Non-CJD controls (100) | 100 | Park et al10 |
| First generation | Full-length hamster | Neuropathologically confirmed sCJD (123) | 89 | MM (90); MV (88); VV (95) | Non-CJD controls (103) | 99 | McGuire et al8 |
| First generation | Full-length hamster | Neuropathologically confirmed sCJD (15) Probable sCJD1 (13) | 87 69 | MM (78); MV (75); VV (100) | Neurodegenerative disease (12) and no neurological disorder (31) controls | 100 | Orrú et al15 |
| First generation | Full-length hamster | Neuropathologically confirmed sCJD (36) Probable sCJD1 (68) | 69 79 | MM (71); MV (66); VV (33) | Non-prion disease controls (64) | 100 | Groveman et al |
| First generation | Full-length hamster | Neuropathologically confirmed sCJD (179) Probable sCJD1 (97) | 83 79 | MM (84); MV (72); VV (80) | Non-CJD controls (915) | 99 | Lattanzio et al12 |
| First generation | Full-length hamster | Neuropathologically confirmed sCJD (24) Probable sCJD1 (26) | 79 65 | ND | No controls | NA | Bongianni et al13 |
| Second generation | Truncated hamster | Neuropathologically confirmed sCJD (48) | 96 | MM (95); MV (100); VV (100) | Neurodegenerative disease controls (30) Controls no neurological disorder (9) | 100 | Orrú et al15 |
| Second generation | Truncated hamster | Neuropathologically confirmed sCJD (36) Probable sCJD1 (68) | 94 97 | MM (94); MV (66); VV (66) | Non-prion disease controls (64) | 100 | Groveman et al 11 |
| Second generation | Truncated hamster | Neuropathologically confirmed sCJD (10) Probable sCJD1 (12) | 70 100 | ND | Non-CJD controls (17) | 100 | Bongianni et al13 |
| Second generation | Truncated hamster | Neuropathologically confirmed sCJD (116) Probable sCJD1 (73) | 92 95 | MM (92); MV (92); VV (98) | Non-CJD controls (100) | 100 | Franceschini et al17 |
| Second generation | Truncated hamster | Neuropathologically confirmed sCJD (174) | 93 | MM (94); MV (94); VV (94) | Non-CJD controls (82) | 98 | Foutz et al16 |
Diagnosis of probable sCJD made according to the WHO criteria.31
MM, methionine; MV, methionine and valine; NA, not available; ND, not done; RT-QuIC, real-time quaking-induced conversion; VV, valine; rPRP, recombinant prior protein; sCJD, sporadic Creutzfeldt-Jakob disease.